This work investigates the efficiency of date palm fiber as a biosorbent to the removal of Cr (VI) from aqueous solution. The effect of four main factors including, the initial pH, initial Cr (VI) concentration, biosorbent dosage, and time were assessed. The results revealed that, the maximum 89 % removal of Cr (VI) at time 50 min, initial pH 3.2, initial Cr (VI) 181 ppm, and biosorbent dosage 0.8 % (w/v) was obtained. The artificial neural network (ANN) model was developed to predict the Cr (VI) removal as a function of the studied variables. A number of data points were considered as test data to validate the model. The results indicated a high accuracy of the ANN modeling in estimating the target variable.