Biology Department, science faculty, urmia university, urmia,Iran The relative inefficacy of alpha-amylase inhibitors in affecting human digestion of starch has been highlighted by recent scientific and public controversy over the commercial sales of so-called starch-blockers or slimming pills. These formulations, which were derived frome powdered kidney beans, were claimed to cause weight loss .alpha amylase and its inhibitors are drug-design targets for the development of compounds for treatment of diabetes Obesity and Hyperlipaemia . Plant alpha-amylase inhibitors show great potential as biotechnological tools to engineer resistance of crop plants against Pests. They are also drug-design targets for treatment of diabetes and digestion disorderes.these inhibitors also known as sensitizing agents contain major allergenscausing baker asthma disease in human. the numerous form of alpha –amylase inhibitors were reported .in this study alpha-amylase inhibitor was extracted from Iranian wheat cultivar (Triticum aestivum v zarrin), precipitated and purified by anion exchange fast protein liquid chromatography .electrophoresis of purified protein showed 0.66 relative mobility .total hydrolytic activity of human salivary and bacillus subtilis alpha –amylase were inhibited 97% and 89.97% respectively by collected purified alpha- amylase inhibitor
