Plant alpha-amylase inhibitors show great potential as drug-design targets for treatment of diabetes and digestion disorderes.These inhibitors also known as sensitizing agents in human. The numerous form of alpha amylase inhibitors was reported. In this study alpha-amylase inhibitor was extracted from Iranian wheat cultivar (Triticum aestivum v zarrin), precipitated and purifi ed by anion exchange fast protein liquid chromatography .Electrophoresis of purifi ed protein showed 0.59 relative mobility .Total hydrolytic activity of human salivary and bacillus subtilis alpha amylase were inhibited 92.o1% and 78.99% respectively by collected purifi ed alpha- amylase inhibitor.
