Adaptation of celles to unfavorable temperature in different organisms is ensured by special biochemical mechanisms affecting variuous aspect of metabolism.Heat shock proteins in plants are only proteins syntheesized during the stress period that thoought to function as molecular chaperone. In this study germination of two winter wheat cultivars(Triticum aestivum L.) in different temperature(0-45°C)were compared . Based on the results, Alamut is resistant and zarrin is sensitive cultivar to cold and heat. by using randomized complete block design seedling of 4 days for 24h or 5h at 43°C (heat) ، -4°C (cold) and 18°C (Control) treated. After these treatments total protein and soluble sugars in leaves of wheat cultivars determined. Cold stress(-4°C/24h ) cause increase of soluble sugars in leaves of two cultivars،while total protein content not significantly changed. Heat stress 43°C cause significantly increase of both soluble sugars and total protein in leaves of two cultivars. electrophoresis of leave proteins show expression of heat shock protins (21،17/50 14/6 and 10 KD) in(43°C/5h ) treatment.
