Background: α-Glucosidase inhibition can significantly prevent glucose uptake after meal, and help controlling adverse effects in diabetics. So, determination of α-Glucosidase inhibitory effect and antioxidant activity of the Haplophyllum acutifolium and Ferula haussknechtii aerial organs was the aim of this study. Methods: Inhibitory effect of hexane extracts from different organs was investigated spectrophotometricaly in several concentrations at 405 nm wavelength using a microplate reader. Antioxidant activity of hexane extracts from various organs was also measured using DPPH scavenging and iron reduction tests. Results: The highest inhibitory activity of F. haussknechtii was observed at the 0.1 g/ml concentration of flower extract (100% inhibition and IC50 = 0.1 μg/ml) and the most inhibitory activity of H.acutifolium, was related to the 1 g/ml concentration of flower extract (100% inhibition and IC50 = 10 μg/ml) and leaf extract (100% inhibition and IC50 = 60 μg/ml). Extract of H. acutifolium flower and leaves showed Non-competitive inhibition pattern and F. haussknechtii flower showed mixed (Competitive-Non-Competitive) inhibitory pattern at 0.001 g/ml and exhibit uncompetitive inhibitory pattern at the 0.1 g/ml. The results of antioxidant potential showed EC50 for F. haussknechtii flower and H. acutifolium leaves equalled 2.37 and 0.96 mg/ml, respectively. Conclusions: The hexane extract of the F. haussknechtii flower, and H. acutifolium flower and leaf organ have a significant inhibitory effect on the activity of α-Glucosidase, DPPH free radical scavenging activities and reducing power. So, they are good resources for extraction of medicinal compounds to control blood level of glucose after meal, in diabetic patients
