Biological control involves the use of beneficial organisms, their genes, and/or products, such as metabolites, that reduce the negative effects of plant pathogens and promote positive responses by the plant. Antagonists belonging to the genus of Trichoderma protect plants and limit pathogen populations under different soil conditions. Bacillus spp. with high population level in environment, resistance to high temperature and formation of resistant spores, are important and safe candidate for biological control. To evaluate the effect ofbiofertilizer (Trichoclermin and subtilin) on the control of Fusarium wilt of chickpea cultivars, an experiment was conducted in a randomized complete design with three replications in green house. Experimental factors were biofertilizer (Trichodermin, Subtilin and both mixing), cultivars (Pirooz, Hashem, ILC- 482) and biofertilizer using method (seed treatment, foliar applications). Results indicated that the effect of biofertilzer were significantly different for cultivars (P