جهانشیر امینی

Some 500 Trichoderma isolates were obtained from soil samples and bark of trees collected from west parts of Kurdistan province. Cultures were grown on PDA and purified on 2% water agar by hyphal tip method prior to identification. The isolates were identified using morphological features, including colony characters (pigmentation and growth rate on PDA) and microscopic characters, including shape of conidiophores, shape and size of conidia and phialides. The microscopic features were studied and recorded 3‐5 days after inoculation on cultures grown on CMD at 25°C under ambient laboratory condition. Nine species identified, including T. citrinoviride, T. longibrachiatum, T. saturnisporum, T. asperellum, T. atroviride, T. harzianum, T.arundinaceum, T. brevicompactum and T.virens, in addition six isolates possess conidiophor similar to species in section Longibrachiatum were morphologicaly different from others and introduced Trichoderma species until now, therefore these isolates can be a new species of Trichoderma. In order to accurate identification and support morphological studies internal transcribed spacers of the rDNA, translation elongation factor 1‐alpha (tef‐1alpha) and a fragment of the gene coding for endochitinase 42 (ech42) these isolated were nucleotide sequenced and compared by using blast search with introduce Trichoderma species. Result of molecular study supported morphological studies and these isolates could be a new species of Trichoderma, belonging to Longibrachiatum section.