Introduction: Volleyball injuries are more common in the lower extremity. Although the knee joint is the second most common volleyball injury, it ranks is first in terms of the severity of the injury. The most common knee ligament injury is ruptured ACL. The ACL injury occurs in 70% to 75% of cases with a non-contact mechanism. The knee valgus is a key risk factor for non-contact ACL injury, so that knee valgus 88% can predict ACL injury in female athletes. The Sports Metrics Volleyball Training (SMVT) is one of the most successful lower extremity injury prevention protocols in the female volleyball player’s. But its effect on the improvement knee alignment and vertical jump in female volleyball player’s whit knee valgus during Drop Vertical Jump (DVJ) test has not been studied. Therefore, this study investigates the effect of the SMVT on improvement knee alignment during DVJ and vertical jump in female volleyball player’s whit knee valgus. Methods: The subjects of study consist of 30 young female volleyball players with knee valgus, which randomly divided into two groups (TG: Training Group, n=15, age:20.4±1.8 years, weight 57.4±6.7 kg, height 166.4±6.3 cm, BMI 20.6±1.6 kg/m2, and experience 5.4±1.9 years) and (CG: Control Group, n=15, age:21.2±2.5 years, weight 60.8±7.8 kg, height 169.9±6.3 cm, BMI 20.9±1.4 kg/ m2, and experience 5.5±1.7 years). The TG performed 6 weeks SMVT and CG continued normal exercises. Pre-test and post-test assessment’s included DVJ and vertical jump test. In order to analyze the statistical data, the SPSS (version 25) and the ANCOVA test (P≤0.05) was used. Results: The analysis of covariance test results revealed that the effects of the Pre-test, as well as the effects of groups on the post-test variables in the TG, were statically significant (P<0.01) for all variables. Additionally, The TG experienced significant reduced (P<0.01) in knee valgus during DVJ, in the maximum knee flexion frame (from 23/1º to 7/8º), and significant increase vertical jump height (from 30.4 to 34 cm). Conclusion: The SMVT is a specialized neuromuscular exercise designed to prevention knee injuries in female volleyball players. Due to the correction key risk factors of noncontact ACL injury (knee valgus), and increased vertical jump performance, it seems that in female volleyball players with knee valgus, the SMVT can help to prevent noncontact knee injuries during functional activities like DVJ, and it can also help to improve performance Therefore, performing SMVT is recommended for female Volleyball player’s whit knee valgus because it is effective in both preventing injuries and significant improvement performance.