The main aim of this study was to optimize the design parameters of the fruit shakers for efficient harvesting of Shengy olive. A single-degree-of-freedom spring-mass model was established to determine the natural frequency and damping coefficient of the limb. A tractor-mounted shaker that transmits vibration to limbs and fruits via a reciprocating mechanism was fabricated for field evaluation of the forced vibration modes. A 3×4 factorial experiment with a completely randomized design was conducted to investigate the effects of shaking amplitudes and frequencies on fruit removal. The shaking mode with a frequency of 10 Hz and amplitude of 80 mm transmitted the average power of 92 W to remove 95% of fruits in the field trial. This oscillation characteristic should be used to redesign the fruit shakers to pass human safety standards and efficient harvesting.