Three new localities of Mediodactylus russowii zarudnyi, (Nikolsky, 1899) were recorded in Iran during field work in north eastern regions of the Iranian Central Desert. Specimens were collected from Tooran Protected Area in Semnan Province, eastern Iran in April 2011 and June 2012. This is the 3rd record of this species from Iran and also the first record of occurrence of Mediodactylus russowii zarudnyi from Semnan Province. The new localities of the species are situated about 350 km west of the old locality and also 670 km west of the type locality. As a result, the distribution range of Mediodactylus russowii zarudnyi has been extended.
