An investigation was done on shoot regeneration via direct organogenesis from leafy petiole explants of Gerbera jamesonii cv. ‘Royal Soft Pink’. Murashige and Skoog (MS) medium was supplemented with 0.1 mg l-1 indole-3-acetic acid (IAA) and additions of various concentrations and combinations of thidiazuron (TDZ) and benzyl adenine (BA). Combinations of TDZ and BA showed significantly enhanced shoot regeneration compared with using TDZ or BA alone. The highest evaluations for percentage of shoot regeneration (85.43 %) and number of shoots per explant (12.88) was recorded in the medium supplemented with 0.1 mg l-1 IAA and 1.0 mg l-1 TDZ plus 4.0 mg l-1 BA. In vitro raised shoots were rooted on MS medium supplemented with 0.5 mg l-1 NAA and plantlets acclimatized successfully in greenhouse conditions.
