Gerbera is known as an important plant in flower industry, and is considered as a favorite floricultural crop worldwide, ranked fourth among the top cut flowers. This research was conducted to investigate the effects of different concentrations of benzyladenine (BA) [1, 2, and 3 mgL-1] solely and or in combinations with naphthalene acetic acid (NAA) [0.2 and 0.4 mgL-1] on the induction, multiplication and proliferation of shoots in Gerbera gamesonii Bolus cv. ‘Royal soft pink’ from shoot tip explants in a Murashige and Skoog (MS) medium. For rooting of shoots three concentrations of NAA (0, 0.5 and 1 mg L-1) were examined. The results showed that the induction and proliferation of shoot were affected by the combination of BA and NAA. The highest number of shoots (10 shoots per explant) after two sub culture were obtained in MS medium supplemented with 2 mgL-1 BA and 0.2 mgL-1 NAA. Considering root fresh and dry weights and root length in rooted plantlets it was not shown the significant difference between 0.5 and 1 mg L-1 NAA for rooting of shoots. The in vitro rooted plantlets were acclimatized and transferred to greenhouse with 80% success.
