In the Mediterranean, unbalanced precipitation distribution causes drought stress in rainfed wheat cultivation. Under such conditions, supplemental irrigation (SI) is a highly efficient practice to reduce the adverse effects of drought stress and improve yield. The present study aimed to assess the effects of SI on traits related to N accumulation and remobilization, chlorophyll concentration, yield and yield components, and water use efficiency (WUE) in different cultivars. Two field experiments with four irrigation levels (I0= rainfed, I1= SI at booting stage, I2= SI at booting and anthesis stages, I3= SI at booting, anthesis, and grain filling stages) and five cultivars (Rejaw, Sardari, Homa, Azar2, and Sirwan) was conducted during the 2015–2016 and 2016–2017 wheat growing seasons.