Ichthyophthirius multifiliis is a ciliated protozoan which can cause Ich disease. This disease is a major problem to aquarists and commercial fish product world wide. The classic sign of an Ich infection is the presence of small white spot on the skin or gills. In present study presence the parasite in parrot fish with white spot on the body were confirmed by microscopic examination of skin and gill. Two infected parrot were transferred to a 70-l aquarium with 20 Angel fish in .After 12 h mortality were started in fish. The eyes in dead fish were cloudy . After disinfection the surface of the eyeball by means of alcohol and red-hot blade eye fluids were collected in clean Petri dishes separately and were checked microscopically by wet mount. Immature forms of parasite, tomites were detected in eye liquids. In the next step gold fish aquariums were infected with eye fluid from angel fish in room temperature(22±2 Cº) .12-48 h post challenge mortality was initiated in exposed gold fish, cloudy eyes was the main sign in the dead fish. Samples of eyes and brain suspension were provided following disinfect the outside of the fish by means of alcohol and red-hot blade. Hemorrhages were evident in brain and immature parasite were observed in eye liquids and brain suspensions
