In this study, TiO2/MCM-41 nanocomposite was prepared by loading 10 wt.% titania onto the mesopores structure of hydrothermally synthesized MCM-41 molecular sieve via impregnation method and was used to remove Tetracycline by adsorption process. The XRD and EDX results revealed that the nanocomposite adsorbent was successfully synthesized. Using BET N2-adsorption technique, the specific surface area of TiO2/MCM-41 was found to be 870 m2/gr. The experimental results demonstrated that TiO2 and neat MCM-41 exhibit very less pollutant adsorption compared to TiO2/MCM-41. It was also found that the nanocomposite adsorbent has excellent adsorptive potential, giving 92 % removal at 50 mg/L Tetracycline concentration and ambient temperature after 5 hrs. From kinetic studies, the adsorption of Tetracycline on TiO2/MCM-41 was found to follow the pseudo-second-order kinetics.