Elicitors could be used as enhancers of plant-secondary-metabolite synthesis and could play an important role in biosynthetic pathways to enhanced production of commercially important compounds. In this work Purslan hairy roots were cultured for 4 weeks in 250 ml Erlen shake flasks containing ½ MS liquid medium, then the effect of different concentrations of Methyl Jasmonate (MJ) elicitor and Salicylic acid (SA) was investigated on accumulative dopamine in these hairy roots. The different concentrations used for MJ were 0 (control) 100,150, 200 Mmol, and for SA were 0 (control) 125,250 and 500 Mmol. When the final growth phase was completed the treatments were added after 48 h of finishing a 28-day period. It is found that different concentrations of MJ, especially 100 Mmol concentration are an important factor regarding the increase of producing dopamine secondary metabolite, so that 100 Mmol concentration makes the dopamine 4.3 fold times more than treatment control. On the other hand, there could be no significant effect of the different concentrations of SA, on dopamine accumulation compared to the control treatments.
