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Bandar Astinchap

Bandar Astinchap

Academic rank: Associate Professor
ORCID:
Education: PhD.
ScopusId: 24342779500
Faculty: Faculty of Science
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Research

Title
Label-free attomolar detection of lactate based on radio frequency sputtered of nickel oxide thin film field effect transistor
Type
JournalPaper
Keywords
Fieldeffecttransistor Lactatebiosensor, NiOthin film, Radiofrequencysputtering, Chitosan,
Year
2017
Journal BIOSENSORS & BIOELECTRONICS
DOI
Researchers mansori majd samira ، Abdollah Salimi ، Bandar Astinchap

Abstract

The radio frequency sputtered nickel oxide thin film nanostrtablucture deposited on glass substrate was used as a potential matrix for the realization of highly sensitive and selective field effect transistor-type lactate biosensor. Firstly, NiO-FET was tested for NADH detection showing a linear concentration range 1 aM to 1 nM and a low detection limit of 0.2 aM. Then, NiO surface modified with chitosan and functionalized with glutaraldehyde and lactate dehydrogenase enzyme was immobilized on the aldehyde terminal. The biosensor is found to exhibit highly efficient sensing response characteristics with good linearity of 1 aM to 1 pM and low limit of detection of 0.5 aM. The biosensor shows high stability without interferences from commonly interfering compounds in biological fluids, including uric acid, ascorbic acid, glucose and acetaminophen. Furthermore, the application of the proposed biosensor for analysis of lactate in artificial serum samples was evaluated with good satisfactory results. This protocol can be used to develop of disposable, low cost, and portable various types of dehydrogenase based biosensor devices using metal oxide nanomaterials.