Background: Cattle are known as the main host in bovine tuberculosis disease caused by Mycobacterium bovis, but the sheep is relatively resistant to this infection and therefore few cases of its disease occur in sheep. Only when cattle and sheep are in very close contact with each other and the abundance of mycobacterium in the environment is very high, sheep infected by this disease. Case Presentation: In the present study, a case of generalized tuberculosis in sheep in the slaughterhouse was reported. Disease diagnosis was done based on necropsy lesions (generalized tuberculous granuloma), Ziehl-Neelsen staining and PCR technique for Mycobacterium bovis. Conclusion: The present report shows that Mycobacterium bovis strains present in the region have the ability to be transmitted to sheep and cause disease with severe clinical symptoms. This report is very important particularly for mixed farming of cattle and sheep.
