Bipolar electrochemistry (BPE) has been lately explored as a simple and reliable electrochemical technique for the adjustment of various conductive substrates. The BPE, composed of two driving electrode along with a bipolar electrode, provides particular advantages compared to conventional electrochemistry especially in a simple setup, which involve one direct current (DC) power supply, low cost, ease of setup, no direct electrical connection is required and many electrodes can be controlled simultaneously with a single DC power supply. Herein, this is applied to derive the gold nanostructures on the surface of bipolar gold electrode. The modified electrode is utilized as a suitable substrate for immobilization of glucose dehydrogenase enzyme aimed at direct electrocatalytic oxidation of glucose as sustainable biofuel in the biofuel cell compartments. The structure and surface morphology of modified electrode were determined by spectroscopic methods such as scanning electron microscopy, atomic force microscopy and electrochemical impedance spectroscopy. Electrocatalytic activity of modified electrode toward oxidation of glucose was investigated by cyclic voltammetry technique. The onset potential and the magnitude of current density for glucose oxidation on the modified electrode were obtained to be -0.03 V (vs. Ag/AgCl) and 2.7 mA cm-2, respectively. This newly developed modified electrode has the potential to be used as bioanode in biofuel cells. In conclusion, results of this study clearly indicate the effectiveness of the proposed method for modifying the metals surfaces with high potential ability designed for extensive catalytic performances, particularly adsorption of biomolecules for electrocatalytic applications.
