Hydrogels are 3D polymeric networks with great swelling capability in water and appropriate chemical, mechanical and biological features which make it feasible to maintain bioactive substances. Herein, we fabricated carbon dots-chitosan nanocomposite hydrogels via reacting carbon dots synthesized from various aldehyde precursors with chitosan after that functionalized with ssDNA probe for detection of microRNA-21 in MCF-7 cancer cells. More importantly, three fluorescent hydrogels were produced using schiff base reaction (forming imine bonds) among the amine in chitosan and aldehyde groups on the CDs surface. Furthermore, the hydrogel films, CDs and CDs-chitosan nanocomposite hydrogels were characterized by UV–vis absorption and fluorescence spectra, FT-IR, scanning electron microscope (SEM) and transmission electron microscopy (TEM). The DNA hydrogel bioassay strategy revealed a great stability and a superb sensitivity for microRNA-21, with a suitable linear range (0.1–125 fM) and a detection limit (0.03 fM). For sample analysis, the biosensors exhibited good linearity with MCF-7 cancer cell concentrations from 1000 to 25000, 1000–25000 and 1000–6000 cells mL 1 and detection limit of 310, 364 and 552 cells mL 1, for glutaraldehyde, nitrobezaldehyde and benzaldehyde based nanocomposite hydrogels, respectively. In addition, cell viability consequences demonstrated low probe cytotoxicity, so nanocomposite hydrogels was utilized to multicolor imaging of MCF-7 cancer cells.
