This study was conducted to measure the bioconcentration of silver nanoparticles (AgNPs) in artemia brine shrimp (Artemia salina) and its transfer to zebrafish (Danio rerio), in order to: (1) determine the uptake of AgNPs by artemia from water, and (2) evaluate trophic transfer potential of AgNPs from artemia to zebrafish under controlled conditions. The artemia exposed to 0.5, 1.0, and 2.0 mgL-1 AgNPs for 24 h and then were administrated to zebrafish for 14 days. Silver body burden was assayed using a Phoenix 886 flame atomic absorption spectrophotometer both in artemia and zebrafish. The results of this study showed that the uptake of AgNPs in artemia was higher than zebrafish (p < 0.05). The accumulation of AgNPs in zebrafish was dose dependent, with greater accumulation observed at higher AgNPs concentrations. Moreover, the results of this study indicated that the trophic transfer factor (BMF) of AgNPs was lower than 1 (< 1), and this nanoparticle was not potential of trophic transfer from artemia to zebrafish.