The study of antibiotics in gene transformation in Chrysanthemum focuses on optimizing the use of antibiotics as selective agents and their impact on plant regeneration within horticultural practices. Antibiotics like carbenicillin, cefotaxime, Clavamox, and kanamycin are evaluated for their effectiveness in eliminating Agrobacterium and promoting healthy plant growth during genetic transformation. Kanamycin, in particular, is widely used as a selective agent to identify transformed cells due to its reliability and efficiency. Research highlights the importance of selecting minimal concentrations of antibiotics to ensure efficient regeneration and transformation. For instance, Clavamox has shown promising results in maintaining plant vigor while effectively replacing other antibiotics. This research contributes to advancing Agrobacterium-mediated transformation techniques, enabling the introduction of desirable traits into Chrysanthemum, such as improved resistance, ornamental qualities, and horticultural value. The findings pave the way for more efficient and sustainable genetic engineering practices in ornamental plant cultivation.
